Biocompatibility assessment of resin-based cements on vascularized dentin/pulp tissue-engineered analogues

A three-dimensional (3D) dentin/pulp tissue analogue, resembling the human natural has been engineered in an vitro setup, aiming to assess cytocompatibility of resin-based dental restorative cements. Stem Cells from Apical Papilla (SCAP) and Human Umbilical Vein Endothelial (HUVEC) were embedded Collagen-I/Fibrin hydrogels at 1:3 ratio within 24-well plates. Hanging culture inserts placed over hydrogels, housing odontoblast-like cell layer a treated-dentin barrier. Shear modulus 3.5 5 mg/ml was evaluated by dynamic mechanical analysis. Eluates two cements, dual-cure- (Breeze™, Pentron: Cement-1/C1), self-adhesive cement (SpeedCEMplus™, Ivoclar-Vivadent: Cement-2/C2) applied into analogue after pre-stimulation with LPS. Cytocompatibility assessed MTT assay, live/dead staining real-time PCR Both hydrogel concentrations showed similar shear moduli pulp until day (D) 7, while mg/ml-hydrogel substantially increased stiffness D14. cements caused no significant toxicity analogue. C1 induced stimulation (p < 0.01) viability (158 ± 3%, 72 h), LPS attenuated this effect. C2 (±LPS) minor reduction (15–20%, 24 h) that recovered h for LPS+ group. upregulation VEGF, ANGP-1, downregulation respective receptors VEGFR-2 Tie-1. good triggered angiogenic response indicating initiation repair responses released xenobiotics.